1. INTENDED USE
Tetracycline Rapid Test is a competitive immunoassay for the
semi-quantitative detection of the presence of Tetracycline residue
in milk. Cut-off: 100 ppb Assay Time: 5 – 10 min Sample: Defatted
2. PRINCIPLE OF THE ASSAY
Tetracycline Rapid Test is based on competitive lateral flow
immunochromatographic assay. The Tetracycline conjugate in the test
zone will capture the immuno-gold (colloid gold- Tetracycline
antibody conjugate), when there is very little dissociative
Tetracycline in the samples. A visible red test band indicates a
negative result when the control line (C zone) shows that the card
is valid. The test band (T zone) will be not visible if
Tetracycline is present in concentration of 100 ppb and above which
explains a positive result.
3. KIT COMPONENT
- 10×foil pouches each contain a cassette and desiccant
- 1×plastic canister containing 10 microwells and a desiccant
- 20×pipettes, 10×centrifugal tubes (1.5 mL)
- Product Manual
4. TEST PROCEDURE
- Bring 2-3 mL of the under tested milk to room temperature
- Collect 1mL of milk sample into a centrifugal tube. Do
centrifugation at 7000 rpm for 3-4 min to separate the fat
- Take out the microwells strip from the plastic canister. Take one
well and tear off the film. Insert the pipette through the fat
layer (5 mm below) to collect the defatted milk towards the mark on
the pipette (200 μL) and transfer it into the well.
- Use another pipette to repeatedly suck and extrude the sample
until all red reagents are completely dissolved. Wait for 1 min.
NOTE: The sensitivity of the strip is positive correlated to the
reaction time. It is suggested to wait for 1-2 min before running
- Take out the cassette from the foil pouch and place it
- Suck the mixture in the well and gradually drip 3 drops into the
assay sample hole “S”.
- Interpret the result in 5 - 10 minutes. Result after 10 minutes
is only considered as reference.