Sample Types Validated:Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Please read this insert completely prior to using the product. This
kit is used to assay Human Beta 2 glycoprotein(B2-GP) on the basis
of the Biotin double antibody sandwich technology. This Elisa kit
takes one-step method with which solutions do not need
diluting,because we simplifies the dilute process by our lab
techniques. This kit is for research only and is not for use in
This kit is used to assay the Beta 2 glycoprotein(B2-GP)in the
sample of Human’s serum, blood plasma and other related biological
This kit uses enzyme-linked immune sorbent assay (ELISA) based on
the Biotin double antibody sandwich technology to assay the Human
Beta 2 glycoprotein (B2-GP). Add Beta 2 glycoprotein(B2-GP)to the
wells, which are pre-coated with Beta 2
glycoprotein(B2-GP)monoclonal antibody and then incubate. After
that, add anti B2-GP antibodies labeled with biotin to unite with
streptavidin-HRP, which forms immune complex. Remove unbound
enzymes after incubation and washing. Add substrate A and B. Then
the solution will turn blue and change into yellow with the effect
of acid. The shades of solution and the concentration of Human Beta
2 glycoprotein (B2-GP) are positively correlated.
Materials supplied in the Test Kit
Chromogen solution A
Chromogen solution B
Coated ELISA plate
12-well * 8 tubes
Washing concentrate (30X)
Seal plate membrane
Anti B2-GP antibodies labeled with biotin
Materials required but not supplied
1. 37 ℃ incubator 2. Standard Enzyme reader
3. Precision pipettes and Disposable pipette tips 4. Distilled
5. Disposable tubes for sample dilution 6. Absorbent paper
1. Before opening the kit kept at the temperature of 2-8℃, it takes
at least 30 minutes to increase naturally to room temperature.
After breaking the seal of ELISA coated-plate, some of the stripes
used should be kept in hermetic bag.
2.When adding samples, sample injector must be used for each time
and should also be frequently checked for its precision to avoid
3. The instruction must be strictly followed while the reading of
ELISA reader must be set as the standard of determining the
4.Pipette tips and seal plate membrane in hand should not be used
more than once in order to avoid cross contamination.
5.All samples, washing concentration and wastes of every kind
should be disposed as infective agent.
6.Other reagents not needed must be packed or covered. Reagents of
different batches must not be mixed and should be used before their
respective validity dates.
7. Substrate B is sensitive to light and therefore should not be
exposed to light for too long.
Manually washing method: Washing by hand: Shake off the liquids in the wells of the ELISA
plate; Lay several bibulous papers on the test bed and pat hard the
ELISA plate several times downward; then inject at least 0.35ml of
diluted washing concentration for 1-2 minutes' soaking. Repeat this
process as needed.
Automatic washing method: Washing by automatic plate washer: If there is an automatic plate
washer, it should only be used in the test when you are quite
familiar with its functions.
Intra-assay Precision (Precision within an assay): 3 samples with
low, middle and high level Human B2-GP were tested 20 times on one
Inter-assay Precision (Precision between assays): 3 samples with
low, middle and high level Human B2-GP were tested on 3 different
plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Contact Rebecca Yan