The dry blood collection card contains chemically treated paper for preservation of RNA &DNA
in the sample. The sample is stabilized preventing microbial or
environmental degradation. It is easy to use.
Dry Blood Collection Card Materials provided: collection card in a foil pouch with & desiccant bag.One
zip-lock bag.&large shipping envelope with instruction printed
on one side.
Dry Blood Collection Card Sampling Technique:
Wash hands before and after the blood collection procedure.
Collect the blood onto the labeled filter paper, using the
1. Cleanse infant’s heel with 70% isopropyl alcohol (use only
rubbing alcohol). Note: Warming the skin-puncture site with a warm
moist cloth, or a heel warming device, for 3 minutes can increase
blood flow through the site.
2. Allow heel to air dry.
3. Using a lancet, or heel incision device, and wearing gloves,
perform the puncture on the plantar surface of the heel. The
puncture should be made to a depth of less than 2.0 mm with a
sterile lancet or incision device.
4. Gently wipe off first drop of blood with sterile gauze or cotton
ball. The initial drop contains tissue fluids that may dilute
5. Wait for formation of large blood droplet; apply gentle pressure
with thumb and ease intermittently as drops of blood form.
6. Gently touch the printed side of the filter paper card to the
blood drop and in one step, allow a sufficient quantity of blood to
soak through and completely fill a pre-printed circle. Do notpress
the filter paper against the puncture site on the heel. Fill each
printed circle with a SINGLE application of blood. Observe both
sides of the filter paper card to assure that blood uniformly
penetrated and saturated the card. Spotting should be done only on
the printed side.
The filter paper must not touch the skin puncture site.
7. Fill the both of the blood spots for the test.
8. All used items should be disposed of in an appropriate biohazard
9. Elevate infant’s foot above the body and apply pressure using
sterile gauze. Do not apply adhesive bandages.
10. Allow blood specimen to AIR DRY THOROUGHLY, on a horizontally level—non-absorbent open surface, such as a
plastic-coated test tube rack—for a minimum of 3 hours at ambient
temperature and away from direct sunlight. Do not stack, heat, or allow touching other surfaces during the
11. Within 24 hours of collection, prepare the dried blood
collection card for shipment to the laboratory by courier or mail.
Only use the mailing or courier envelope provided. Do not use
plastic or plasticene envelopes. Humidity and moisture are
detrimental to stability of dried blood spot specimens and can
12. The sealed envelope should be placed in a mailing envelope that
is ready with mailing address to a designated laboratory
Contact Rebecca Yan