A rapid, one step test for the qualitative detection of Benzodiazepines in human urine.
For healthcare professionals including professionals at point of
For in vitro diagnostic use only.
Format: Strip, Cassette, Cup,and Panel
The BZO One Step Benzodiazepines Test Strip is a lateral flow
chromatographic immunoassay for the detection of Oxazepam (major
metabolite) in urine at a cut-off concentration of 300 ng/mL. This
test will detect other Benzodiazepines, please refer to Analytical
Specificity table in this package insert.
This assay provides only a preliminary analytical test result. A
more specific alternate chemical method must be used in order to
obtain a confirmed analytical result. Gas chromatography/mass
spectrometry (GC/MS) is the preferred confirmatory method. Clinical
consideration and professional judgment should be applied to any
drug of abuse test result, particularly when preliminary positive
results are used.
The BZO One Step Benzodiazepines Test Strip is an immunoassay based on the
principle of competitive binding. Drugs which may be present in the
urine specimen compete against the drug conjugate for binding sites
on the antibody.
During testing, a urine specimen migrates upward by capillary
action. Benzodiazepines, if present in the urine specimen below
cut-off, will not saturate the binding sites of the antibodies in
the test strip. The antibody coated particles will then be captured
by immobilized benzodiazepine-protein conjugate and a visible
colored line will show up in the test line region. The colored line
will not form in the test line region if the benzodiazepines level
exceeds cut-off concentration, because it will saturate all the
binding sites of anti-benzodiazepines antibody.
POSITIVE: One colored line appears in the control region (C). No line appears in the test region (T). This positive result
indicates that the Benzodiazepines concentration exceeds the
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques
are the most likely reasons for control line failure. Review the
procedure and repeat the test with a new test strip. If the problem
persists, discontinue using the test kit immediately and contact
your local distributor.
1. The BZO One Step Benzodiazepines Test Strip provides only a qualitative,
preliminary analytical result. A secondary analytical method must
be used to obtain a confirmed result. Gas chromatography/mass
spectrometry (GC/MS) is the preferred confirmatory method. 1,2
2. It is possible that technical or procedural errors, as well as
other interfering substances in the urine specimen may cause
3. Adulterants, such as bleach and/or alum, in urine specimens may
produce erroneous results regardless of the analytical method used.
If adulteration is suspected, the test should be repeated with
another urine specimen.
4. A Positive Result does not indicate level or intoxication,
administration route or concentration in urine.
5. A Negative Result may not necessarily indicate drug-free urine.
Negative results can be obtained when drug is present but below the
cutoff level of the test.
6. Test does not distinguish between drugs of abuse and certain
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