The myoglobin and creatine kinase MB parts are the main markers for
the early diagnosis of myocardial infarction (AMI), but may be
non-specific for clinical diagnostic purposes. The troponin is the
specificity marker; the level of cTnI in blood is abnormal during 4
h after the onset of chest pain was moderate and will peak in 8-16
h. The rising level could be tested within 6-10days. Myo,CK-MB,
cTnI mass concentration are sensitive markers for the diagnosis of
The rapid test kit is fast and sensitive for an in vitro
qualitative detection of Myo, CK-MB and cTnI in human whole blood,
which is for the early and differential diagnosis of myocardial
infarction in emergency, intensive care unit, cardiac medicine,
health posts and other institutions acute, provides an objective,
comprehensive, efficient new means clinical diagnosis of myocardial
The test is based on the principle of double antibody sandwich
immunoassay for the qualitative detection of Myo,CK-MB, cTnI in
human serum. Three cards were Myo, CK-MB and cTnI test strip. Each
test strip has the control line C (Control) and the test line
(Test), the control line coated with anti-mouse antibody and the
test line was coated with anti-Myo, CK-MB and cTnI monoclonal
antibodies. When specimen is added to sample pad, it moves through
the conjugate pad and mobilizes gold Myo/CK-MB/cTnI conjugate that
is coated on the conjugate pad. The mixture moves along the
membrane by capillary action and reacts with Myo/CK-MB/cTnI
antibody that is coated on the test region. If Myo/CK-MB/cTnI is
present, the result is the formation of a colored band in the test
region. If there is no Myo/CK-MB/cTnI in the sample the area will
The detection thresholds were: Myo: 80ng/ml; CK-MB: 5ng/ml; cTnI:
Test cards, disposable plastic dropper, instructions
Requirements on Specimen
1. Whole blood for testing, if collect pls test asap.
Keep the test strip and the specimen back to room temperature If
not ready, do not tear foil bag,
1. Remove the strip from the foil bag.
2. Add 80-100μl whole blood into the three specimen holes on the
test card separately..
3. Read the test results within 20 minutes. Do not interpret
results after 20 minutes.
Positive: Two red band appears in the test area (T), another in the
Negative: Only control line (C) a red band appears in the detection
zone (T) without the red band appears.
Invalid: Quality control area (C) does not appear red band,
indicating that the operator error or reagent failure.
This kit provides qualitative results only, and cannot determine
the exact content of three cardiac markers. A patient suffering
from acute myocardial infarction, physicians should be combined
with clinical features and diagnosis of acute myocardial infarction
symptoms and other Comprehensive judgments.
1. Check whether the packaging is complete, it cannot be used if
the contents are damaged.
2. The test card is used only for in vitro rapid diagnosis.
3. All specimens and the test cards and used the dropper might be a
potential risk, should be considered infectious waste for
incineration and other treatment.
4. Specimens cannot add anticoagulants, not tender a glass tube
full bloom, carefully observe the test line, a faint line as long
as there is positive to prevent undetected.
Storage and Expiry
Store at room temperature 4-30 ℃, avoid hot and sunshine, dry
place, not frozen, valid for 24 months. This kit can be short-term
room temperature transport, in summer and winter should take
certain protective measures to avoid heat or freezing and thawing.
Contact Rebecca Yan